Inoculate Colonized Mushroom Grains To mushroom substrates

Discover the proper way to inoculate bulk mushroom substrates with fresh colonized grain spawn. In order to successfully mix the best grain spawn bags into hardwood mushroom bulk substrate fallow these practices. Begin by preparing a clean workspace with 70% isopropyl alcohol to prevent contamination. Merry Mushroom Maidens advises you use a laminar flow hood or a sterilized glove box. Gather your mushroom growing supplies: hardwood substrate, mycelium grain, and suitable tools like a small scoop. Open your mushroom grow bag and introduce your mycelium grain spawn to the prepared hardwood substrate inside and seal. It's crucial to ensure that the spawn grains you are adding are fully colonized with mycelium because it prevents contamination. Gently break apart any clumps of grain spawn to facilitate an even distribution throughout the bulk substrate. For optimal results, use a mixing ratio of around 1:10 mycelium grains to hardwood substrate and a 1:1 ration for cubensis mushrooms by volume. Aim for a consistent texture where the grains are evenly dispersed within the substrate. Incubate the mushroom grow bag in a dark, warm environment at 70-80 degrees to allow the mycelium to further colonize the hardwood and CVG bulk substrate. Keep an eye out for any contamination. In a few weeks, you should see white mycelium spreading through the substrate, indicating successful colonization. Make sure your hardwood and CVG bulk substrate block is fully colonized with white, healthy, mycelium. Before preparing to grow your specific variety of mushrooms and you unsure? Any questions? You can email or call us anytime during business hours.